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Production of Biodiesel Feedstock from Lignocellulosic Biomass by
Oleaginous Fungi and Yeasts Program of Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90110 Thailand Lignocellulosic biomass are attractive feedstocks for production of biofuel due to their low cost, renewable nature and abundance. This study aimed to produce lipids as biodiesel feedstocks from lignocellulosic biomass by oleaginous fungi and yeasts. Oleaginous fungi could pretreat biomass and simultaneously produce lipids through solid state fermentation (SoSF). After pretreatment, the cellulose content in biomass was increased by 1.27 folds and the fungi also produced lipids at 147-169 mg/g-biomass. The fungal pretreated biomass was used for lipid production by the yeast in separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF). The yeast gave the maximum lipid yield of 46.7±1.5 mg/g-pretreated biomass through SHF and 52.8±1.26 mg/g-pretreated biomass through SSF. In order to combine the process of enzyme production and lipid production, the fungi was co-cultured with the yeast and this process gave comparable lipid yield of 32.5± 8.7 mg/g-pretreated biomass. The fungal-yeast lipids are composed of palmitic acid, oleic acid, linoleic acid, and stearic acid, which are suitable as biodiesel feedstocks. This study has shown that the promising process for bioconversion of biomass into biodiesel feedstocks. |
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